Thermo Fisher Scientific PI3K p85 alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_8526641) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p85 alpha,uniProtId:P27986-1,ncbiNodeId:9606,antigenRange:240-380,antigenLength:724,antigenImageFileName:PA5-88089_PI3K_p85_alpha_P27986-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88089_PI3K_p85_alpha_P27986-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.56 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804642

Product Specific Information

Positive Samples: U-251MG, Jurkat, SKOV3, mouse brain, mouse liver, rat brain, rat liver

Immunogen sequence: LQYLLKHFFK LSQTSSKNLL NARVLSEIFS PMLFRFSAAS SDNTENLIKV IEILISTEWN ERQPAPALPP KPPKPTTVAN NGMNNNMSLQ DAEWYWGDIS REEVNEKLRD TADGTFLVRD ASTKMHGDYT LTLRKGGNNK L

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.