Thermo Fisher Scientific Myeloperoxidase Monoclonal Antibody (SP72)
Human myeloperoxidase 단백질을 인식하는 rabbit monoclonal antibody (clone SP72). Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. Protein A 정제, PBS/BSA buffer에 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Myeloperoxidase Monoclonal Antibody (SP72)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1:100 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP72 |
| Immunogen | Synthetic peptide corresponding to C-terminus of human myeloperoxidase protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.212 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537902 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single-chain precursor and subsequently cleaved into light and heavy chains.
The mature MPO is a tetramer composed of two light and two heavy chains. This enzyme produces hypohalous acids that are central to neutrophil microbicidal activity.
Diseases associated with MPO include Myeloperoxidase Deficiency and Familial Alzheimer’s Disease type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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