Thermo Fisher Scientific IRF4 Monoclonal Antibody (SP114)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Flow Cytometry (Flow)

1:230

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP114

Immunogen

A synthetic peptide near C-terminus of human MUM1/IRF4 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IRF4,uniProtId:Q15306-1,ncbiNodeId:9606,antigenRange:451,antigenLength:451,antigenImageFileName:MA5-16382_IRF4_Q15306-1_Rabbit.svg,antigenImageFileNamePDP:MA5-16382_IRF4_Q15306-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.102 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537901

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Hodgkin`s lymphoma.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.