Thermo Fisher Scientific IRF4 Monoclonal Antibody (SP114)
IRF4 단백질을 인식하는 Rabbit monoclonal antibody (Clone SP114). Western blot, IHC, Flow cytometry에 사용 가능. Human 시료 반응성. Protein A/G 정제, PBS 기반 buffer에 1% BSA 함유. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 |
| Flow Cytometry (Flow) | 1:230 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP114 |
| Immunogen | A synthetic peptide near the C-terminus of human MUM1/IRF4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.102 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537901 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM EDTA buffer for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is Hodgkin’s lymphoma.
Target Information
The IRF (interferon regulatory factor) family of transcription factors is characterized by a unique tryptophan pentad repeat DNA-binding domain.
IRFs regulate interferon expression in response to viral infection and control interferon-inducible genes.
IRF4 is lymphocyte-specific and negatively regulates toll-like receptor (TLR) signaling, which is central to innate and adaptive immune activation.
A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may cause multiple myeloma.
Alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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