Thermo Fisher Scientific Mesothelin Monoclonal Antibody (SP74)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.32 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP74

Immunogen

Synthetic peptide corresponding to C-terminus of human mesothelin protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Mesothelin,uniProtId:Q13421-1,ncbiNodeId:9606,antigenRange:630,antigenLength:630,antigenImageFileName:MA5-16378_Mesothelin_Q13421-1_Rabbit.svg,antigenImageFileNamePDP:MA5-16378_Mesothelin_Q13421-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537897

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is mesothelioma.

Target Information

Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. While the biological functions of both Mesothelin and MPF remain speculative, high-levels of expression in cancerous tissues compared to limited distribution in normal tissues strongly suggests their involvement in tumorigenesis. Both have been demonstrated to promote tumor cell proliferation, migration,anchorage-independent growth, and tumor progression; demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.