Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), eFluor 660, eBioscience

Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (Clone 429), eFluor 660

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 429 monoclonal antibody reacts with mouse CD106 (VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation.
CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), playing a key role in lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests involvement in inflammatory responses.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤ 0.5 µg/test, 100 µL final volume). Optimal titration recommended.

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647. Emits at 659 nm, excited at 633 nm (red laser).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may play roles in inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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