Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), eFluor 660, eBioscience
마우스 CD106(VCAM-1)을 인식하는 단클론 항체로, eFluor 660 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 염증 반응 및 세포 부착 관련 연구에 활용됩니다. 0.2 mg/mL 농도의 액상 형태로 제공되며, 4°C에서 암소 보관합니다.
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Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (Clone 429), eFluor 660
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 429 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219864 |
Available Formats
- Unconjugated
- APC
- Biotin
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The 429 monoclonal antibody reacts with mouse CD106 (VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation.
CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), playing a key role in lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests involvement in inflammatory responses.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤ 0.5 µg/test, 100 µL final volume). Optimal titration recommended.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647. Emits at 659 nm, excited at 633 nm (red laser).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may play roles in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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