Thermo Fisher Scientific CD3/HLA Class 2 Antibody Cocktail, FITC, PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1/IgG2a

Class

Cocktail

Type

Antibody

Clone

UCHT1/WR18

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome, Human B-CLL cells

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538433

Product Specific Information

MA5-16958 contains a monoclonal mixture of FITC-conjugated CD3 (clone #UCH-T1, Mouse IgG1) and RPE-conjugated HLA Class 2 (clone #WR18, Mouse IgG2a). UCHT1 recognizes the human CD3 cell surface antigen expressed by T lymphocytes. WR18 recognizes a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes. This antibody is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes.

Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.