Thermo Fisher Scientific Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Recombinant Rabbit Monoclonal Antibody (OSP/8693R)
Osteopontin(SPP1) 단백질을 인식하는 재조합 토끼 단일클론 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 적합함. HEK293 발현, Protein A/G 정제, PBS buffer, 1 mg/mL 농도. 뼈 형성 및 세포 부착 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Functional Assay (Functional) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Dog, Human, Mink, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | OSP/8693R |
| Immunogen | Recombinant full-length rat Spp1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates OPN function and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and serving as substrates for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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