Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Super Bright 702, eBioscience

Applications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 585
• NovaFluor Yellow 590, 660, 730
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and is effective in treating renal, heart, and liver allograft rejection. CD3 subunits (gamma, delta, epsilon) are essential for TCR assembly and surface expression. CD3 is expressed by thymocytes and all mature T cells.

Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.5 µg)/test.
Excitation: 405 nm
Emission: 702 nm
Laser: Violet Laser

Super Bright 702 is a tandem dye excited by the violet laser (405 nm) and emits at 702 nm. Use a 710/50 bandpass filter. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded on chromosome 11. They interact with cytoplasmic signal transduction molecules through tyrosine-based motifs.

CD3 antigen is found on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells. It is absent on B and NK cells. Reduced T cell percentages may occur in autoimmune diseases. Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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