Thermo Fisher Scientific Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Monoclonal Antibody (OSP/4589)
Osteopontin 단백질을 인식하는 Rat monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Avian, Dog, Human 등 다양한 종에 반응. Protein A/G 정제, 4°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Functional Assay (Functional) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Dog, Human, Mink, Mouse, Rabbit, Rat |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OSP/4589 |
| Immunogen | Recombinant full-length rat Spp1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and cellular transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates its function and integrin-binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and serving as a substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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