Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (M148)
Phospho-STAT5 (Tyr694) 단일클론 항체로 인간, 마우스, 랫트 시료에 반응. Western blot 및 ICC/IF에 적합. EGF 처리 세포에서 92 kDa Stat5A 밴드 검출. 고순도 친화 크로마토그래피 정제, 안정적 액상 형태로 연구용 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M148 |
| Immunogen | Clone (M148) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942624 |
Product Specific Information
The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
STAT5 alpha is activated by ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and is essential for tumorigenesis.
The mouse counterpart of STAT5 alpha induces expression of BCL2L1/BCL-X(L), suggesting its antiapoptotic function. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues of virgin and lactating mice, though differential accumulation has been reported in muscle and mammary tissue. STAT5 alpha plays critical roles in reproduction, lactation, immune function, and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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