
Thermo Fisher Scientific Phospho-BAD (Ser75) Polyclonal Antibody
Phospho-BAD (Ser75) 항체로, 인간 BAD 단백질의 Ser75 인산화 부위를 인식하는 rabbit polyclonal antibody입니다. Western blot 및 IHC(P)에서 검증되었으며, 세포사멸 조절 관련 연구에 적합합니다. 단백질 A 정제, PBS buffer, 0.09% sodium azide 함유.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser75 of human Bad |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2061986 |
Target Information
The protein encoded by BAD is a member of the BCL-2 family, which regulates programmed cell death. BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, reversing their anti-apoptotic activity. Its proapoptotic function is regulated by phosphorylation, involving kinases such as AKT and MAP kinase, and phosphatase calcineurin. Alternative splicing results in two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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