Thermo Fisher Scientific DLL4 (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sDLL-4. Anti-Human sDLL-4-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hsDLL-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sDLL-4 (500-P279) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hsDLL-4.

Western Blot:
To detect hsDLL-4 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hsDLL-4 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

500-P279BT-1MG will be provided as 2 × 500 µg.

Target Information

Delta-like ligand 4 (DLL4), also known as Delta-4, is a homologue of the Drosophila delta protein and functions as a transmembrane bound ligand to the Notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF) as a negative feedback regulator to control angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 is strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer. Inhibition of DLL4 results in increased vascular proliferation but defective maturation, leading to decreased tumor growth without apparent toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.