Thermo Fisher Scientific DLL4 (soluble) Polyclonal Antibody, PeproTech

Thermo Fisher Scientific DLL4 (soluble) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sDLL-4. Anti-Human sDLL-4-specific antibody was purified by affinity chromatography employing an immobilized Human sDLL-4 matrix.

Sandwich ELISA:
To detect Human sDLL-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Human sDLL-4 (500-P279Bt) as a detection antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Human sDLL-4.

Western Blot:
To detect Human sDLL-4 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant Human sDLL-4 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Delta-like ligand 4 (DLL4), also known as Delta-4, is a homologue of the Drosophila delta protein and functions as a transmembrane-bound ligand to the Notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF) as a negative feedback regulator to control angiogenic sprouting and promote formation of a differentiated vascular network.

DLL4 is strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer. Inhibition of DLL4 leads to increased vascular proliferation but defective maturation, resulting in decreased tumor growth without apparent toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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