Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, PerCP-eFluor 710, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

0.5 µg/test

Product Specifications

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

View additional formats

• [Agarose](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Agarose/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 488-FluoroNanogold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-FluoroNanogold/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 594-FluoroNanogold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 594-FluoroNanogold/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/APC/host/Goat/)
• [bGal](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/bGal/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [DyLight 800 4X PEG](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 800 4X PEG/host/Goat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/eFluor 570/host/Goat/)
• [eFluor 660](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/eFluor 660/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Goat/)
• [Functional Grade](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Functional Grade/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Red 685](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Red 685/host/Goat/)
• [NovaFluor Red 700](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Red 700/host/Goat/)
• [NovaFluor Yellow 590](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 590/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/PE/host/Goat/)
• [Qdot 525](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 525/host/Goat/)
• [Qdot 565](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 565/host/Goat/)
• [Qdot 585](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 585/host/Goat/)
• [Qdot 605](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 605/host/Goat/)
• [Qdot 655](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 655/host/Goat/)
• [Qdot 705](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 705/host/Goat/)
• [Qdot 800](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 800/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2573754

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab`)2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.

This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.

Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.