Thermo Fisher Scientific FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor Plus 594
인간 FOXP3 단백질을 인식하는 단클론 항체로 Alexa Fluor Plus 594 형광 표지. IHC(P) 등 다양한 응용에 사용 가능하며, 고순도 친화 크로마토그래피 정제. CD4+CD25+ Treg 세포 연구에 적합.
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Thermo Fisher Scientific FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor Plus 594
Applications and Tested Dilution
- Immunohistochemistry (Paraffin) (IHC (P)): 20 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCH101 |
| Conjugate | Alexa Fluor™ Plus 594 |
| Excitation / Emission Max | 590 / 618 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
Product Specific Information
The PCH101 antibody reacts with the amino terminus of human FOXP3 protein (also known as FORKHEAD BOX P3, SCURFIN, JM2). Cross-reactivity with other proteins has not been determined.
FOXP3, a 49–55 kDa protein, belongs to the forkhead/winged-helix family of transcriptional regulators and is the gene defective in scurfy (sf) mice. Constitutive high expression of FOXP3 mRNA is observed in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression in CD4+CD25− cells imparts a Treg phenotype.
Intracellular staining of human PBMCs with PCH101 antibody using the anti-human FOXP3 staining set reveals approximately 0.5–4% of lymphocytes staining, predominantly in the CD25^bright population (donor variability dependent).
PCH101 cross-reacts with rhesus, chimpanzee, and cynomolgus species.
Applications Reported: Immunoblotting (WB), immunohistology on frozen and paraffin-embedded tissue sections.
Applications Tested: IHC of formalin-fixed paraffin-embedded tissue using high pH antigen retrieval (20 µg/mL recommended). Antibody should be titrated for optimal assay performance.
Using conjugate solutions: Centrifuge briefly before use and use only the supernatant to minimize nonspecific background staining.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and highly expressed in CD25+CD4+ regulatory T cells. Defects in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome in humans (immune dysfunction, polyendocrinopathy, enteropathy, X-linked). FOXP3 expression is regulated by p53 in response to DNA damage in human breast and colon cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- Alexa Fluor Plus 647 available
- Custom conjugation upon request
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