Thermo Fisher Scientific CD11c Monoclonal Antibody (118/A5), Alexa Fluor Plus 647

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

118/A5

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Description: The monoclonal antibody 118/A5 recognizes human CD11c, a 150 kD type I transmembrane protein which is also known as integrin alpha X. Integrins are heterodimeric integral membrane proteins made up of an alpha and a beta chain and are involved in cell adhesion. The CD11/CD18 adhesion molecules consist of three heterodimers sharing a common beta subunit (CD18) with a distinct alpha subunit (CD11a, CD11b, CD11c). The CD11c/CD18 complex is expressed on dendritic cells (highly expressed on type 1 myeloid DCs), monocytes, macrophages, granulocytes, NK cells, and subsets of T and B cells. CD11c is also expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias. CD11c plays a role in adhesion and CTL killing through its interactions with CD54, iC3b and fibrinogen.

Applications Reported: This 118/A5 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This 118/A5 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.