Thermo Fisher Scientific CD11c Monoclonal Antibody (118/A5), Alexa Fluor Plus 647

Applications and Tested Dilution

Product Specifications

Product Specific Information

The monoclonal antibody 118/A5 recognizes human CD11c, a 150 kD type I transmembrane protein also known as integrin alpha X. Integrins are heterodimeric integral membrane proteins composed of alpha and beta chains involved in cell adhesion.
The CD11/CD18 adhesion molecules consist of three heterodimers sharing a common beta subunit (CD18) with distinct alpha subunits (CD11a, CD11b, CD11c). The CD11c/CD18 complex is expressed on dendritic cells (especially type 1 myeloid DCs), monocytes, macrophages, granulocytes, NK cells, and subsets of T and B cells.
CD11c is also expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias. It plays a role in adhesion and CTL killing through interactions with CD54, iC3b, and fibrinogen.

Applications Reported:
This antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections, and microscopy.

Applications Tested:
Validated for IHC (P) with high pH antigen retrieval at 20 µg/mL. Optimal performance should be determined by titration.

Handling Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to minimize nonspecific background from aggregates.

Target Information

CD11, together with CD18, forms a heterodimeric adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, and eosinophils.
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, and granulocytes.
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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