Thermo Fisher Scientific beta-Arrestin 1,2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

3 µg/mL

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Fruit fly, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C D(384) D I V F E D F A R L R L K(397) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta-Arrestin 1,2,uniProtId:P49407-1,ncbiNodeId:9606,antigenRange:384-397,antigenLength:418,antigenImageFileName:PA1-730_beta-Arrestin_1,2_P49407-1_Rabbit.svg,antigenImageFileNamePDP:PA1-730_beta-Arrestin_1,2_P49407-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2274371

Product Specific Information

PA1-730 detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin.

PA1-730 has been successfully used in Western blot, Immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.

The PA1-730 immunizing peptide corresponds to amino acid residues 384-397 from human beta-arrestin2. This peptide (Cat. # PEP-156) is available for use in neutralization and control experiments.

Target Information

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.