Thermo Fisher Scientific beta-Arrestin 1,2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-730 detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin.

PA1-730 has been successfully used in Western blot, Immunohistochemistry (paraffin), and Immunoprecipitation procedures.
By Western blot, this antibody detects approximately 49 kDa and 47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.

The PA1-730 immunizing peptide corresponds to amino acid residues 384–397 from human beta-arrestin2.
This peptide (Cat. # PEP-156) is available for use in neutralization and control experiments.

Target Information

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system.
Serving as an adaptor or scaffold molecule, Beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor).

After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues.
Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane.
Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.