Thermo Fisher Scientific beta-Arrestin 1,2 Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 사용 가능한 폴리클로날 항체. 인간 및 랫드 베타-아레스틴1,2 인식. 항원 친화 크로마토그래피로 정제된 고순도 항체. -20°C 보관, 반복 동결/해동 피해야 함.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific beta-Arrestin 1,2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 3 µg/mL | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Fruit fly, Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic Peptide: C D(384) D I V F E D F A R L R L K(397) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2274371 |
Product Specific Information
PA1-730 detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin.
PA1-730 has been successfully used in Western blot, Immunohistochemistry (paraffin), and Immunoprecipitation procedures.
By Western blot, this antibody detects approximately 49 kDa and 47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.
The PA1-730 immunizing peptide corresponds to amino acid residues 384–397 from human beta-arrestin2.
This peptide (Cat. # PEP-156) is available for use in neutralization and control experiments.
Target Information
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system.
Serving as an adaptor or scaffold molecule, Beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor).
After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues.
Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane.
Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.