Thermo Fisher Scientific Phospho-EIF2S1 (Ser52) Polyclonal Antibody
인간, 마우스, 효모 등 다양한 종에 반응하는 phospho-EIF2S1 (Ser52) 폴리클로날 항체. Western blot, IHC, ICC 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 포맷으로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 62 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 2 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
| Inhibition Assays (IA) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Yeast |
| Published Species | Chicken, Fruit fly, Human, Mouse, Non-human primate, Rat, Tag, Virus, Yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF2α that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly, and yeast. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533736 |
Target Information
EIF2α (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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