Thermo Fisher Scientific RIC8A Monoclonal Antibody (OTI3G3), TrueMAB
RIC8A 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공되며 연구용으로 사용됩니다.
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Thermo Fisher Scientific RIC8A Monoclonal Antibody (OTI3G3), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G3 |
| Immunogen | Full length human recombinant protein of human RIC8A produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
RIC8A is a guanine nucleotide exchange factor (GEF) that activates specific G-alpha proteins such as GNAI1, GNAO1, and GNAQ, but not GNAS.
It regulates microtubule pulling forces during mitosis by stimulating G(i)-alpha protein activity, influencing the release of G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex.
It also enhances G(q)-coupled receptor-mediated ERK activation via GNAQ.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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