Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PerCP-Cyanine5.5, eBioscience
마우스 NK1.1 항원을 인식하는 PK136 단클론 항체로, PerCP-Cyanine5.5 형광체로 결합되어 NK 세포 및 특정 T 세포 검출에 적합합니다. Flow Cytometry에 최적화되어 있으며, 0.5 µg/test 사용 권장. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PerCP-Cyanine5.5, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_914359 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB.
Common laboratory strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For detection of NK cells in these strains, use monoclonal antibody DX5 (Product #14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by most cells, with small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on C57Bl/6 mouse splenocytes; ≤0.5 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal performance.
Excitation: 488 nm
Emission: 695 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161) is expressed by Natural Killer (NK) cells, which mediate cytotoxicity and cytokine secretion after immune stimulation.
KLRB1 protein contains extracellular C-type lectin motifs, a transmembrane domain, and a cytoplasmic domain.
NKR-P1A (CD161), encoded by KLRB1, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.
Classified as a type II membrane protein due to its external C terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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