Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PerCP-Cyanine5.5, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB.
Common laboratory strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For detection of NK cells in these strains, use monoclonal antibody DX5 (Product #14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by most cells, with small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on C57Bl/6 mouse splenocytes; ≤0.5 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal performance.

Excitation: 488 nm
Emission: 695 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161) is expressed by Natural Killer (NK) cells, which mediate cytotoxicity and cytokine secretion after immune stimulation.
KLRB1 protein contains extracellular C-type lectin motifs, a transmembrane domain, and a cytoplasmic domain.
NKR-P1A (CD161), encoded by KLRB1, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.
Classified as a type II membrane protein due to its external C terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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