Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Red 700, Yellow 610
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright series (436–780)
• Request custom conjugation available

Product Specific Information

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21–25 kDa protein (Gr-1), a GPI-linked myeloid differentiation antigen. Gr-1 is expressed in bone marrow granulocytes and peripheral neutrophils, serving as a marker for these populations.
Testing indicates RB6-8C5 also stains cells expressing high Ly6C levels. For Ly-6G-specific detection, use clone 1A8-Ly6G (Product #9668).

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells ≤0.06 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration for best performance.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Granulocytes are white blood cells containing cytoplasmic granules, also known as polymorphonuclear leukocytes (PMN). Neutrophil granulocytes are the most abundant type. Granulocytes are released from bone marrow under complement protein regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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