Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.25 µg/test
• Publications: View 221 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, Biotin
• Brilliant Violet 421, 605, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat FOXP3 (FORKHEAD BOX P3, SCURFIN, JM2). FOXP3 is a 49–55 kDa transcriptional regulator associated with CD4+CD25+ regulatory T cells (Treg).
Immunoblotting with FJK-16s mapped the epitope to amino acids 75–125 of mouse FOXP3. In humans, this region can be alternatively spliced; both isoforms are expressed in CD4+CD25+ lymphocytes.

This antibody is optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00). Use of other fixation and staining buffers is not recommended.

Applications Reported: Intracellular staining followed by flow cytometry.
Applications Tested: Intracellular staining of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol.
Recommended use: ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator crucial for immune homeostasis. It is highly expressed in CD25+CD4+ regulatory T cells, low in CD4+CD25− cells, and absent in CD4−CD8+ T cells.
FOXP3 defects cause the scurfy phenotype in mice and are linked to IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans.
Transcript variants encoding different isoforms are identified. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지