Thermo Fisher Scientific TNF beta Chimeric Recombinant Rabbit Monoclonal Antibody (3F12.2D3)
사람 TNF beta를 인식하는 recombinant rabbit monoclonal antibody입니다. Western blot, Flow cytometry, ELISA 등 다양한 응용에 사용 가능합니다. 고순도 Protein A 정제 제품으로 안정적인 결과를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| ELISA (ELISA) | Assay-dependent |
| Neutralization (Neu) | Assay-dependent |
| Surface Plasmon Resonance (SPR) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3F12.2D3 |
| Immunogen | 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LT-alpha expressed in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074514 |
Product Specific Information
Specificity:
3F12 binds to human LT-alpha-3 and LT-alpha-Beta in both its soluble homotrimeric and membrane heterotrimeric forms.
Preparation:
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LT-alpha expressed in E. coli. The hybridoma clone was selected for its ability to bind LT-alpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mouse mAb 2G7 to create the chimeric version of the antibody.
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. It mediates a wide range of inflammatory, immunostimulatory, and antiviral responses, contributes to the formation of secondary lymphoid organs during development, and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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