Thermo Fisher Scientific TNF beta Chimeric Recombinant Rabbit Monoclonal Antibody (3F12.2D3)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Neutralization (Neu)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3F12.2D3

Immunogen

3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LT-alpha expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074514

Product Specific Information

Specificity: 3F12 binds to human LT-alpha-3 and LT-alpha-Beta in both its soluble homotrimeric and membrane heterotrimeric forms.

Preparation: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.

Target Information

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.