Thermo Fisher Scientific Aldolase Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

ELISA (ELISA)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

1:500-1:5,000

-

Product Specifications

Species Reactivity

Human, Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% gentamicin sulfate

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].

Target Information

Aldolase encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.