Thermo Fisher Scientific TIGIT Chimeric Recombinant Rabbit Monoclonal Antibody (4D4)
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TIGIT 단백질을 특이적으로 인식하는 재조합 토끼 단클론 항체. Flow Cytometry, ELISA 등 다양한 분석에 사용 가능. 고순도 Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용, 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific TIGIT Chimeric Recombinant Rabbit Monoclonal Antibody (4D4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 0.17 ng/mL |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4D4 |
| Immunogen | Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by s.c. and foot pad immunization and booster injections. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074565 |
Product Specific Information
- Specificity: Clone 4D4 specifically recognizes TIGIT.
- Preparation: Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and foot pad immunization and booster injections.
Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium, and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
Target Information
TIGIT binds with high affinity to the poliovirus receptor (PVR), leading to increased secretion of IL10 and decreased secretion of IL12B. This interaction suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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