Thermo Fisher Scientific IL-10 Chimeric Recombinant Rabbit Monoclonal Antibody (9D7)

Applications

Product Specifications

Immunogen

Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates.

Product Specific Information

• Specificity: The six 9D7 Fab CDR loops interact with a discontinuous IL-10M1 epitope comprised of residues 71–83 and 125–137. 9D7 non-competitively antagonises IL-10R1 binding.
• Preparation: Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653 is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, blocks NF-kappa B activity, and regulates the JAK-STAT signaling pathway. Knockout studies in mice indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are associated with HIV-1 susceptibility and rheumatoid arthritis. Related diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.