Thermo Fisher Scientific BCKDHA Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 BCKDHA 단백질 인식 토끼 폴리클로날 항체. Western blot 및 ICC/IF에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein of human BCKDHA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720161 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction represents the second major step in the catabolism of branched-chain amino acids. The BCKD complex comprises three catalytic components: a heterotetrameric (α₂β₂) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2), and a homodimeric dihydrolipoamide dehydrogenase (E3).
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, and the reaction is irreversible, constituting the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also includes two regulatory enzymes: a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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