Thermo Fisher Scientific IP3 Receptor 2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: E(317) L N P D Y R D A Q N E G K T V R D(334). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IP3 Receptor 2,uniProtId:P29995-1,ncbiNodeId:10114,antigenRange:317-334,antigenLength:2701,antigenImageFileName:PA1-904_IP3_Receptor_2_P29995-1_Rabbit.svg,antigenImageFileNamePDP:PA1-904_IP3_Receptor_2_P29995-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2130127

Product Specific Information

PA1-904 detects inositol 1,4,5-trisphosphate receptor type-II (IP3R-II) from rat and mouse tissues.

PA1-904 has been successfully used in immunohistochemistry, immunofluorescence and immunocytochemistry procedures. Immunohistochemical staining of IP3R-II in rat brain with PA1-904 results in staining of the hippocampus, corpus collosum, and cerebellum. This product fails to detect IP3R-II in Western blot procedures.

PA1-904 immunizing peptide corresponds to amino acid residues 317-334 from rat IP3R-II. PA1-904 immunizing peptide (Cat. # PEP-024) is available for use in neutralization and control experiments.

Target Information

Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to IP3R, IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. IP3R type I (IP3R-I) has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II is found predominantly in the brain. IP3R-III is known to be expressed in pancreatic islets, kidney, and the gastrointestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.