Thermo Fisher Scientific BPDE-DNA Monoclonal Antibody (5D11)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody recognizes BPDE-I-DNA (PAH-DNA).

Flow Cytometry:
Samples were fixed in 2% paraformaldehyde and permeabilized with 0.2% Triton X-100 / 0.1% sodium citrate. Samples were treated with proteinase K and RNase. DNA denaturation was performed using 4N HCl. Samples were blocked with 5% normal serum before incubation with the monoclonal antibody.

ELISA:
Plates were coated with 50 ng/well BPDE-DNA in 50 mM Tris-buffer (pH 7.5) overnight at 4°C. Plates were blocked with 1% FCS. DNA samples (4 µg) were mixed with 5D11 and added to wells. Detection was performed using a Goat anti-Mouse IgG (AP) for 90 min at 37°C.

IHC (P):
5 µm sections were treated with RNase and proteinase K. DNA was denatured with 4N HCl and neutralized with 50 mM Tris base. Sections were blocked with 1.5% normal horse serum prior to staining.

Target Information

Polycyclic aromatic hydrocarbons (PAHs) are known to bind to DNA, leading to DNA damage that can occur early and late in the malignant process, acting as both initiators and promoters of tumor progression. PAHs are released into the environment through incomplete combustion of organic materials, such as smoking or grilled foods. Human exposure to PAHs can occur via occupational, environmental, dietary, and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) are useful for identifying PAH-DNA adducts, which are believed to be carcinogenic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.