Thermo Fisher Scientific E2F3 Monoclonal Antibody (PG30)
E2F3 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot 및 Immunoprecipitation에 사용 가능. 인간 시료 반응성. 단백질 A로 정제된 액상 항체이며, 4°C 단기 보관 및 -20°C 장기 보관 권장. 연구용 전용 제품.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 4–8 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PG30 |
| Immunogen | Recombinant human E2F3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.3 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2293325 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors.
The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins.
It is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain conserved domains including:
- DNA binding domain
- Dimerization domain (for interaction with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain within the transactivation domain
E2F3, along with E2F1 and E2F2, has an additional cyclin-binding domain and binds specifically to retinoblastoma protein (pRB) in a cell cycle–dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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