Thermo Fisher Scientific LAP2 Monoclonal Antibody (6E10)
Thermo Fisher Scientific의 LAP2 Monoclonal Antibody (6E10)는 인간 LAP2 단백질을 인식하는 mouse IgG1 항체로, WB, ICC/IF, ChIP 등 다양한 연구용 분석에 적합합니다. 고순도 정제된 액상 형태로 제공되며, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ChIP assay (ChIP) | 2.5 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6E10 |
| Immunogen | Synthetic peptide corresponding to residues G(29)EQRKDVYVQLYLQHLTARNRP(50) of human LAP2 (common to all isoforms) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_794754 |
Product Specific Information
- Recommended positive control: K562
- MA1-25323 is expected to cross-react with mouse (100% conserved), rat (100% conserved), Xenopus laevis (90% conserved), and bovine (88% conserved) due to sequence homology.
Target Information
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes defining the border between the nucleus and cytoplasm. These membranes are joined by nuclear pore complexes, allowing molecular exchange. The inner membrane is lined by the nuclear lamina composed of lamins. Lamins attach to the inner nuclear membrane through Lamin Associated Polypeptides (LAPs). LAP1A, 1B, and 1C share sequence homology, while LAP2 is distinct. LAP1A/B bind lamins A, C, and B1; LAP2 binds lamin B1 and also interacts with chromosomes. Phosphorylation of LAP2 during mitosis inhibits these interactions, indicating its role in nuclear envelope reassembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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