Thermo Fisher Scientific LAP2 Monoclonal Antibody (6E10)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6E10

Immunogen

Synthetic peptide corresponding to residues G(29) E Q R K D V Y V Q L Y L Q H L T A R N R P(50) of human LAP2 (common to all isoforms). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LAP2,uniProtId:P42166-1,ncbiNodeId:9606,antigenRange:29-50,antigenLength:694,antigenImageFileName:MA1-25323_LAP2_P42166-1_House_mouse.svg,antigenImageFileNamePDP:MA1-25323_LAP2_P42166-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794754

Product Specific Information

Recommended positive controls: K562.

MA1-25323 is expected to cross react with mouse (100% conserved), rat (100% conserved), Xenopus laevis (90% conserved), and bovine (88% conserved) due to sequence homology.

Target Information

In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A/B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.