Thermo Fisher Scientific MSLN Monoclonal Antibody (OTI5B12), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Mesothelin was initially identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas. It is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein mainly expressed by mesothelial cells.
While normal expression is limited to pleural, pericardial, and peritoneal membranes, aberrant overexpression occurs in several cancers including ovarian, pancreatic, endometrioid uterine, and squamous cell carcinomas of the esophagus, stomach, lung, and cervix.

Proteolytic cleavage of Mesothelin yields a soluble fragment known as megakaryocyte potentiating factor (MPF), which can stimulate megakaryocyte colony formation in murine bone marrow cell cultures. MPF may play a role in megakaryocyte proliferation and differentiation, influencing platelet production.

High expression levels of Mesothelin and MPF in tumor tissues compared to normal tissues suggest their involvement in tumorigenesis, promoting tumor cell proliferation, migration, and metastasis through heterotypic cell adhesion mechanisms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.