Thermo Fisher Scientific Mek2 Polyclonal Antibody
MEK2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 0.5 mg/mL 액상 시약. 핵, 세포질, 세포골격에 국소화된 양성 대조군 3T3 세포 추천.
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Thermo Fisher Scientific Mek2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from internal segment of human Mek2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3
- Cellular location: localizes to nucleus, cytoplasm and cytoskeleton
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction. MEK2 (MAPKK2) is part of the tyrosine/threonine protein kinase family that activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, forming a negative feedback loop that suppresses MEK1 activation.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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