Thermo Fisher Scientific Cytokeratin 6 Monoclonal Antibody (SP87)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:200

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP87

Immunogen

Synthetic peptide derived from C-terminus of human keratin 6 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 6,uniProtId:P02538-1,ncbiNodeId:9606,antigenRange:564,antigenLength:564,antigenImageFileName:MA5-16373_Cytokeratin_6_P02538-1_Rabbit.svg,antigenImageFileNamePDP:MA5-16373_Cytokeratin_6_P02538-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin 6,uniProtId:P48668-1,ncbiNodeId:9606,antigenRange:564,antigenLength:564,antigenImageFileName:MA5-16373_Cytokeratin_6_P48668-1_Rabbit.svg,antigenImageFileNamePDP:MA5-16373_Cytokeratin_6_P48668-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Cytokeratin 6,uniProtId:P04259-1,ncbiNodeId:9606,antigenRange:564,antigenLength:564,antigenImageFileName:MA5-16373_Cytokeratin_6_P04259-1_Rabbit.svg,antigenImageFileNamePDP:MA5-16373_Cytokeratin_6_P04259-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537892

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate cancer or skin tissue.

Target Information

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.