Thermo Fisher Scientific Hexa-histidine (Epitope Tag) Monoclonal Antibody (6HIS, 3550)
6x His 태그 단백질 검출용 마우스 단클론 항체. Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용 가능. Hexa-histidine 태그 단백질 특이적 결합. 연구용으로 단백질 정제 및 검출에 적합.
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Thermo Fisher Scientific Hexa-histidine (Epitope Tag) Monoclonal Antibody (6HIS, 3550)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Immunoprecipitation (IP) | 2–4 µg/500 µg of protein |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6HIS, 3550 |
| Immunogen | Hexa-histidine tagged human CD70 recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins lacking specific antibodies by fusing a known epitope to the target protein.
His-tagged proteins are purified via metal chelate chromatography due to the strong affinity of histidine residues to metal ions such as Ni²⁺.
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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