Thermo Fisher Scientific Hexa-histidine (Epitope Tag) Monoclonal Antibody (6HIS, 3550)
6x His 태그 단백질 검출용 마우스 단클론 항체로, Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합. Hexa-histidine 태그 단백질에 특이적이며, 고순도 Protein A/G 정제. 연구용 단백질 검출 및 정제에 활용 가능.
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Thermo Fisher Scientific Hexa-histidine (Epitope Tag) Monoclonal Antibody (6HIS, 3550)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Immunoprecipitation (IP) | 2–4 µg/500 µg of protein |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6HIS, 3550 |
| Immunogen | Hexa-histidine tagged human CD70 recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins for which no specific antibody is available by fusing a known epitope to a recombinant protein.
His-tagged proteins are purified using metal chelate chromatography due to the strong and selective affinity of histidine clusters for Ni²⁺ ions, enabling purification to homogeneity on Ni²⁺-NTA adsorbents.
His tag-specific antibodies, such as this product, are used to facilitate detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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