Thermo Fisher Scientific Hexa-histidine (Epitope Tag) Monoclonal Antibody (6HIS, 3550)
6xHis 태그 단백질 검출용 마우스 단일클론 항체. Western blot, ICC/IF, Flow cytometry, IP, IM 등 다양한 응용에 적합. 비결합형 액상 형태로 제공되며, 고순도 Protein A/G 정제. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| Immunoprecipitation (IP) | 2–4 µg/500 µg of protein |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6HIS, 3550 |
| Immunogen | Hexa-histidine tagged human CD70 recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly attached to the N- or C-terminal regions of recombinant proteins to facilitate purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of recombinant proteins for which specific antibodies are unavailable by fusing a known epitope to the target protein.
His-tagged proteins can be purified efficiently due to the strong affinity between histidine clusters and metal ions such as Ni²⁺.
His tag-specific antibodies are widely used for detection, purification, and co-immunoprecipitation of His-tagged recombinant proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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