Thermo Fisher Scientific Bacillus anthracis Spore Antigen Monoclonal Antibody (SA27)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

SA27

Immunogen

Bacillus anthracis spore extract.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.8 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557710

Product Specific Information

The Bacillus anthracis spore antigen molecular weight is ~93 kDa.

Target Information

Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.