Thermo Fisher Scientific ANGPT2 Monoclonal Antibody (OTI3H7), TrueMAB
Human ANGPT2 단백질(aa 19-496)을 인식하는 Mouse IgG1 단클론 항체로, WB 및 IHC(P) 검증 완료. TrueMAB 형식의 고순도 항체로, 재조합 단백질 기반 면역원 사용. 연구용으로 혈관신생 관련 단백질 분석에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 19–496 of human ANGPT2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ANG-2 binds to the endothelial cell-specific receptor Tie-2, but unlike ANG-1, does not induce tyrosine phosphorylation.
It modulates ANG-1 activation of Tie-2 and can act as either an agonist or antagonist of Tie-2–induced angiogenesis depending on the environment.
ANG-1, ANG-2, and Tie-2 interactions are essential for embryonic and adult angiogenesis.
ANG-2 is involved in sprouting, tube formation, and maintaining structural integrity of new blood vessels.
Mature human ANG-2 is a secreted protein with 480 amino acid residues, composed of an alpha-helix-rich coiled coil N-terminal domain and a fibrinogen-like C-terminal domain, existing mainly as a disulfide-linked dimer.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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