Thermo Fisher Scientific c-Myc Monoclonal Antibody (9E10)
Thermo Fisher Scientific의 c-Myc Monoclonal Antibody (9E10)는 인간 및 생쥐 시료에 반응하며 다양한 응용(서던 블롯, IHC, ICC 등)에 사용 가능. 0.5 mg/mL 농도의 정제된 액상 제품으로, PBS 완충액에 0.1% sodium azide 포함. 연구용으로 세포 증식 및 종양 관련 c-Myc 단백질 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | 92 |
| Immunohistochemistry (IHC) | – | 2 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 3 |
| Immunocytochemistry (ICC/IF) | 2–10 µg/mL | 20 |
| Flow Cytometry (Flow) | – | 3 |
| ELISA | 0.1–1.0 µg/mL | 4 |
| Immunoprecipitation (IP) | 2–5 µg | 19 |
| ChIP assay (ChIP) | 5 µg/mL | 5 |
| Functional Assay | – | 1 |
| Gel Shift (GS) | Assay-dependent | – |
| Affinity Purification (AP) | – | 1 |
| GST Pull Down (GPD) | – | 1 |
| Miscellaneous PubMed | – | 4 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bacteria, Bovine, C. elegans, Dog, Fruit fly, Hamster, Human, Mouse, Mycetozoan, Pig, Plant, Protozoa, Rat, Xenopus, Yeast |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E10 |
| Immunogen | A 32 amino acid synthetic peptide (aa 408–439) derived from the C-terminus of the human c-Myc protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533008 |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- DyLight 488
- FITC
- HRP
- Request custom conjugation
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with cell proliferation and cycle regulation.
c-Myc is essential for vasculogenesis and angiogenesis during tumor development. The c-myc oncogene regulates normal cellular proliferation and differentiation, and deregulated expression induces apoptosis in various cell types. Antibodies against c-myc epitopes recognize Myc-tagged proteins fused to amino- or carboxy-termini.
Amplification of the c-Myc gene is found in multiple human tumors including lung, breast, and colon carcinomas. N-Myc amplification occurs in neuroblastomas. Translocation of the c-myc locus to immunoglobulin loci is characteristic of Burkitt’s lymphoma and other B-cell proliferative disorders. Aberrant c-myc expression is observed in tumors of diverse origins including colorectal, gastric, hepatic, mammary, ovarian, thyroidal, and hematopoietic neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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