Thermo Fisher Scientific AP2 alpha Polyclonal Antibody
AP2 alpha 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 Immunocytochemistry에 적합. HeLa nuclear extract를 양성 대조로 권장하며, 다양한 종에서 교차 반응 예상. 고농축 액상 형태로 제공되며 장기 보관 시 -20°C에서 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to residues S(420)HTDNNAKSSDKKEEKHRK(437) of human AP2 alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_779266 |
Product Specific Information
- Recommended positive controls: HeLa nuclear extract
- PA1-25327 is expected to cross react with mouse (94% conserved), rat (94% conserved), chicken (88% conserved), and ovine (94% conserved) due to sequence homology.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes.
AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in eye, face, body wall, limbs, and neural tube development.
They also suppress genes including MCAM/MUC18, C/EBP alpha, and MYC.
TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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