Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), iFluor 555, THE

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1–4 µg/mL

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• HRP
• iFluor 488
• iFluor 647
• Request custom conjugation available

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus or inserted in an internal region of the protein to facilitate detection and purification.

An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins. THE™ V5 Tag Antibody [iFluor 555], mAb, Mouse is THE™ V5 Tag Antibody (A01724) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3–6. It is suitable for detecting the expression level of V5-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 555.

Target Information

Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or poorly immunogenic proteins when protein-specific antibodies are unavailable.

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is typically used with all 14 amino acids (GKPIPNPLLGLDST), though shorter sequences (IPNPLLGLD) are also used.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.