Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E, 2545)
CD73 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, 인간 시료 반응성. WB, IHC, ICC, Flow Cytometry 등 다양한 면역 분석에 사용 가능. 고순도 Protein A/G 정제, 액상 형태로 제공되며 연구용으로 적합.
- 카탈로그번호
- 4907-MSM5-P1ABX
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NT5E, 2545 |
| Immunogen | Recombinant full-length human NT5E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD73 (Ecto-5′-nucleotidase, 5′-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5′-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
It consists of a dimer of two identical 70 kDa subunits linked to the plasma membrane via a glycosyl phosphatidylinositol anchor. CD73 serves as a lymphocyte differentiation marker and its deficiency is associated with various immunodeficiency diseases.
Other 5′ nucleotidase forms in cytoplasm and lysosomes differ by substrate affinity and ion requirements. The CD73 gene is localized to chromosome 6q14–q21.
Defects in CD73 can lead to joint and arterial calcification and intestinal tuberculosis. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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