Thermo Fisher Scientific IDH2 Recombinant Rabbit Monoclonal Antibody (8H26L4)
Thermo Fisher Scientific의 IDH2 Recombinant Rabbit Monoclonal Antibody (8H26L4)은 인간 IDH2 단백질을 표적으로 하는 고특이성 항체입니다. Western blot 및 ICC/IF에 최적화되어 있으며, 재조합 기술로 생산되어 로트 간 일관성과 높은 감도를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H26L4 |
| Immunogen | Protein corresponding to human IDH2 [aa41–aa452] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2734812 |
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The process involves cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening individual clones to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses: one utilizing NAD and the other NADP as electron acceptors.
Five isocitrate dehydrogenases have been reported:
- Three NAD-dependent isocitrate dehydrogenases (mitochondrial matrix)
- Two NADP-dependent isocitrate dehydrogenases (one mitochondrial, one cytosolic)
The IDH2 gene encodes the NADP-dependent isocitrate dehydrogenase found in mitochondria, playing a role in intermediary metabolism and energy production. This protein may interact with the pyruvate dehydrogenase complex.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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