Thermo Fisher Scientific PKC theta Monoclonal Antibody (M217)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

M217

Immunogen

Clone (M217) was generated from a recombinant mouse PKC theta protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKC theta, and has low homology to other PKC family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.25 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942658

Product Specific Information

This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCtheta on SDS-PAGE immunoblots of human Jurkat cell lysates.

Target Information

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.