Thermo Fisher Scientific p130Cas Monoclonal Antibody (M144)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:250

-

Immunocytochemistry (ICC/IF)

1:250

-

Immunoprecipitation (IP)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M144

Immunogen

Clone M144 was generated from a recombinant protein containing amino acid residues in the C-terminal region of rat p130 Cas. This rat sequence is highly conserved in human, mouse, and chicken p130 Cas.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.0625 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942656

Product Specific Information

The antibody detects a 130 kDa* protein corresponding to the molecular mass of p130 Cas on SDS-PAGE immunoblots of human A431, endothelial, and Hct116 cells.

Target Information

The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.