Thermo Fisher Scientific eNOS Monoclonal Antibody (M221)
eNOS 단백질을 검출하기 위한 Mouse Monoclonal Antibody (Clone M221). Western blot, IHC(F), ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 반응성 보유. 고순도 Protein A 정제, 안정적 PBS/BSA/glycerol 저장 버퍼 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M221 |
| Immunogen | Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acid residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942655 |
Product Specific Information
The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission, and cytotoxicity can all be potentiated through cellular response to NO.
NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidation of L-arginine to produce L-citrulline and NO.
Two constitutive isoforms—brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III)—and one inducible isoform (iNOS, type II) have been cloned.
All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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