Thermo Fisher Scientific NUMB Monoclonal Antibody (OTI2G1), TrueMAB
Human NUMB 단백질을 표적으로 하는 Mouse IgG2a 단일클론 항체로, Western blot에 적합합니다. TrueMAB 포맷으로 제작되어 높은 특이성과 재현성을 제공합니다. 동결건조 형태로 보존되며, 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–365 of human NUMB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein NUMB homolog is an evolutionarily conserved protein involved in the differentiation of neuronal progenitor cells.
NUMB functions in clathrin-dependent endocytosis through interaction with the AP-2 complex and is expressed in postmitotic neurons, contributing to axonal growth.
Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domains show extensive neurite outgrowth, which can be inhibited by voltage-gated Ca²⁺ channel inhibitors or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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