Thermo Fisher Scientific SPI-B Monoclonal Antibody (235D), eBioscience
인간 SPI-B 단백질을 인식하는 235D 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. 고순도(>90%)로 정제되었으며, 4°C에서 보관 가능합니다. 연구용으로만 사용됩니다.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SPI-B Monoclonal Antibody (235D), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 235D |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865455 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The 235D monoclonal antibody recognizes human SPI-B, a member of the ETS transcription factor family involved in lymphoid development and activity. SPI-B binds the PU box (5-GAGGAA-3) and is expressed in mature B cells, immature T cells, plasmacytoid dendritic cells, and intestinal microfold (M) cells. In human tonsil tissue, high SPI-B expression is observed in plasmacytoid dendritic cells and lower levels in germinal center B cells.
Applications Reported:
This antibody is suitable for western blotting, immunohistochemistry (FFPE), microscopy, and immunocytochemistry.
Applications Tested:
Validated for IHC on formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval. Recommended concentration ≤5 µg/mL. Optimal dilution should be determined experimentally.
Target Information
Spi-B is a transcription factor belonging to the ETS family, characterized by a conserved ETS domain for specific DNA binding. It interacts with IRF4, CREBBP, EP300, SPI1/PU.1, JUN, and TBP. Spi-B binds to the PU-box (5-GAGGAA-3), acting as a lymphoid-specific enhancer. Two isoforms are known. Spi-B is primarily expressed in plasmacytoid dendritic cells (pDCs), promoting their development and antiviral response. It is not expressed in T cells or granulocytes but may be present in liver stem cells and B cells, where it plays roles in BCR signaling and B-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.