Thermo Fisher Scientific SMURF1 Monoclonal Antibody (1A9H4)
SMURF1 단백질을 인식하는 Mouse 단일클론 항체로 Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. Recombinant Human SMURF1(198-374AA)을 면역원으로 제작. 고순도 Protein G 정제, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A9H4 |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198–374AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3091102 |
Target Information
Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, while activin and TGFB receptors (e.g., ACVR1 and TGFBR1) target SMAD2 and SMAD3. Phosphorylation of these SMADs induces their association with SMAD4.
Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is essential for maintaining the transformed morphology and motility of tumor cells. Atypical protein kinase C-zeta (PKCζ), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions to control local RhoA levels. Smurf1 links the polarity complex to RhoA degradation in lamellipodia and filopodia, preventing RhoA signaling during dynamic membrane movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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